In vitro flowering – A system for tracking floral organ development in Dendrocalamus hamiltonii Nees et Arn. ex Munro.
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Date
2014-08
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Abstract
Dendrocalamus hamiltonii plants are slender and tall (15-25 m) thereby, rendering tagging, sampling and tracking the development of flowers difficult. Therefore, a reproducible system of in vitro flowering was established for tracking the stages of flower development. MS medium supplemented with 2.22 µM 6-benzylaminopurine, 1.23 µM indole-3-butyric acid and 2% sucrose was optimized as the flower induction medium (FIM) wherein 28 and 42 days were required for the development of gynoecium and androecium, respectively. Six distinct stages of in vitro flower development were identified, and the flowers were comparable with that of in planta sporadic flowers. Pollen viability of the in vitro flowers was higher than those of in planta ones. The in vitro system developed in the present study facilitates easy tracking of different stages of flower development under controlled environmental conditions. It can also be used for medium- or long-term storage of pollens and manipulation of in vitro fertilization.
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Keywords
Bamboo, Flower induction medium, In vitro flowers, Somatic embryo derived plants
Citation
Kaur Devinder, Thapa Pooja, Sharma Madhu, Bhattacharya Amita, Sood Anil. In vitro flowering – A system for tracking floral organ development in Dendrocalamus hamiltonii Nees et Arn. ex Munro. Indian Journal of Experimental Biology. 2014 Aug; 52(8): 825-834.