Effect of different forms of acute stress in the generation of reactive oxygen species in albino wistar rats.
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Date
2014-07
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Abstract
Stress is common for all organisms either in the form of eustress (beneficial) or as distress (harmful). Stress
is highly diverse in present world and its effects in organisms are well studied. This study is a comparative
effect of stress in the generation of reactive oxygen species in albino Wistar rats, which are seldom
reported. Here animals were grouped into four and the test animals of each group were administered with
any one of the following stress, namely forced swimming induced stress (FS), and noise induced stress
(NS), immobilization stress (IS) and overcrowding stress (OS). After stress administration, serum cortisol
was estimated as a bio marker of stress in the albino rats, and the liver homogenate were used to estimate
superoxide dismutase (SOD) by using rat Elisa kit in the spectrophotometer. The data were processed with
unpaired‘t’ test. The cortisol levels were found to be increased in a highly significant level (P<0.001) in all
the groups as compared to the normal control. And the stress level was found to be maximum in the FS
group in comparison with other groups. The mean cortisol level in different stress groups such as FS, NS,
IS and OS were found to be 4.15, 3.7, 3.63, 3.62 μ gm/dl respectively. Among all the stressed groups, the
SOD level in the FS group were found to be increased in a highly significant level (P<0.001) in comparison
with normal control group. The SOD level in FS group was (30.75 U/mgm. protein) followed by OS (28.96),
noise (28.88) and IS (28.77).
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Smitha K K, Mukkadan J K. Effect of different forms of acute stress in the generation of reactive oxygen species in albino wistar rats. Indian Journal of Physiology and Pharmacology. 2014 Jul-Sept; 58(3): 228-231.