Screening of antibacterial activity, qualitative and quantitative analysis of phytochemicals in vitex trifolia.
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Date
2014-06
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Abstract
The present focus of the study was to evaluate the antibacterial activity and phytochemical analysis in the medically important plant vitex trifolia leaf
extract by using solvents like aqueous, methanol, acetone, benzene, petroleum ether. The plant vitex trifolia used for its phytochemical and pharmacological studies.
In qualitative and quantitative analysis of vitex trifolia viz., alkaloids, saponin, tannin, phenols, terpenoids, flavonoids, steroids, were determined using standard
methods. Vitex trifolia leaves were collected, air-dried and soxhlet extracted by using standard method. These extracts were then tested for antimicrobial activity
using disc diffusion method. Vitex trifolia showed highest antibacterial activity (IZ= 0.5cm in acetone extract, IZ = 0.75 in ethanol extract) against Bacillus subtilis
and (IZ= 0.15cm in acetone extract, IZ = 0.25cm in ethanol extract, IZ = 0.35cm in aqueous extract) against Escherichia coli. Results concluded that the
phytochemicals of leaves extract of vitex trifolia has the potential to act as a antibacterial agent.
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Keywords
Vitex trifolia, Antibacterial activity, Zone of inhibition, Muller hinton agar
Citation
Rajakan Nancy Immaculate Mary, Balakrishnan Meenashree, Karthikeyan Rajalakshmi, Jagadheesan Vasanthi. Screening of antibacterial activity, qualitative and quantitative analysis of phytochemicals in vitex trifolia. International Journal of Research and Development in Pharmacy and Life Sciences. 2014 Jun-Jul; 3(4): 1085-1088.