Screening and identification of cellulase producing bacteria isolated from oil palm meal.
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Date
2014-04
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Abstract
Oil palm (Elaeis guineensis) meal, a by-product of palm oil, is rich in fiber and contains lignocelluloses, which
inhibits the absorption of the nutrients has been widely used for animal feed. The improvement of the nutrient
absorption is required treating with cellulase enzyme. This study was aimed to isolate, screen and characterize the
cellulase producing bacteria. Ten strains of cellulolytic bacteria were isolated from 7 oil palm meal samples
collected in Phetchaburi, Prachuap Khiri Khan and Pattani provinces, Thailand. They exhibited the ability to
degrade carboxymethyl-cellulose (CMC) based on the decolorization of CMC-basal agar medium using Congo
red as a color indicator. They showed the cellulase hydrolysis capacity ranged from 1.56 to 4.14. All isolates
were Gram positive rod-shaped bacteria and belonged to Bacillus (8 isolates), Paenibacillus (1 isolate) and
Lysinibacillus (1 isolate) based on the phenotypic characteristics and 16S rRNA gene sequence analysis. Their
cellulase activity ranged from 0.039±0.002 to 0.233±0.005 IU/ml when they were cultivated in broth.
Description
Keywords
Cellulolytic bacteria, cellulase, oil palm meal, Bacillus, Paenibacillus, Lysinibacillus
Citation
Khianngam Saowapar, Pootaeng-on Yupa, Techakriengkrai Taweesak, Tanasupawat Somboon. Screening and identification of cellulase producing bacteria isolated from oil palm meal. Journal of Applied Pharmaceutical Science. 2014 Apr; 4(4): 90-96.