Applications of Micellar Liquid Chromatography in Bioanalysis: A Review.
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Date
2014-01
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Abstract
Micellar liquid chromatography (MLC) is one of the very important analytical techniques. It contains mobile
phase added with surfactants above its critical micellar concentration and the stationary phase is modified with
surfactant monomers. So, micelles alter the solubilising capability of the mobile phase which forms diverse
interactions with major implications in retention and selectivity. It is an alternative to conventional reversed
phase liquid chromatography. It allows direct injection of physiological fluids, analysis of pharmaceutical
compounds, physiological partitioning process. MLC (micelle liquid chromatography) has proved time saving as
compared to other analytical technique like HPLC and ion-pairing. Applicability of MLC is increased in the field
of bioanalysis. It is used to analyze different samples of drugs in serum, urine, food etc. In this review we have
focused on the various examples of use of MLC in bioanalysis. This review also contains basic information about
MLC such as micellar mobile phase, stationary phase, surfactants, and fundamental studies such as retention
behavior and partition coefficient.
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Keywords
MLC, bioanalysis, micelles, partition coefficient and retention behavior
Citation
Kalyankar T M, Kulkarni P D, Wadher S J, Pekamwar S S. Applications of Micellar Liquid Chromatography in Bioanalysis: A Review. Journal of Applied Pharmaceutical Science. 2014 Jan; 4(1): 128-134.