Studies Involving a Commercial Extract of Three Ballerina: ii- Evaluation of the in Vitro Effect on the Labeling of Blood Constituents of rats with Technetium-99m and on the Morphology of the Red Blood Cell.
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Date
2013-12
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Abstract
The aim of this work is to characterize (electric conductivity and refractive index) a Three Ballerina (TB) and to
evaluate its in vitro effect on the labeling of blood constituents with 99mTc and on the morphology of the red
blood cells (RBC). Anticoagulated whole blood (Wistar rat) was incubated with a TB extract and the labeling of
the blood constituents with technetium-99m (99mTc) was performed. Plasma (P) and blood cells (BC) were
isolated and aliquots were also precipitated with trichloroacetic acid to separate soluble (SF) and insoluble
fractions (IF). The %ATI in these samples was calculated. The morphology of the treated RBC showed no
shape’s qualitative alterations. The TB extract was characterized with an electric conductivity of
1.35±0.04mSv/cm and refractive index of 2.21±0.15%BRIX. TB extract decreased significantly (p<0.05) the
radioactivity distribution in the cellular compartment from 96.97±1.30% to 88.48±7.13%, and in IF-P from
74.29±4.12 to 14.26±5.73%. In conclusion, our data show some physical chemical parameters that could be
suitable to characterize the preparation of an extract of TB. Moreover, substances present in the TB extract
should probably have an effect on transport of the ions through the RBC membrane and/or should have redoxi
properties and the stannous ion would decrease and could justify the effect on the fixation of the radioactivity on
the plasma proteins. Moreover, although our experiments were carried out with animals, it is suggested
precaution in the interpretation of the examinations that use labeled blood constituents in patients who are
undergone TB extract.
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Keywords
blood constituents, technetium-99m, red blood cell, Three ballerina, electric conductivity, refractive index
Citation
Pinto Nelson de Souza, Amorim Lúcia de Fátima, Paiva Dulciane Nunes, Barreto Vilma Gomes, Diré Gláucio, Santos-Filho Sebastião David, Bernardo-Filho Mario. Studies Involving a Commercial Extract of Three Ballerina: ii- Evaluation of the in Vitro Effect on the Labeling of Blood Constituents of rats with Technetium-99m and on the Morphology of the Red Blood Cell. Journal of Applied Pharmaceutical Science. 2013 Dec; 3(12): 83-88.