Anti-diabetic effects of ethyl acetate and n-butanol fractions of Acacia nilotica leaves methanolic extract on alloxan-induced diabetic wistar rats.
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Date
2013-11
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Abstract
Evaluation of hypoglycaemic activity of ethyl acetate and n-butanol fractions of Acacia nilotica on alloxan
induced diabetic Wistar rats has been investigated. Two doses of the ethylacetate fraction 50 and 100 mg/kg was
administered. As regard to 50mg/kg caused a significant (P<0.05) reduction in the blood glucose levels when
compared with control at 3,5,7,9 and 12 days of treatment with percentage glycaemia change of 49.1,54.8,60.5,
58.8 and 69.7 respectively. However, the dose of 100mg/kg ,there was a significant decrease (p<0.05) at 3 5 7, 9
and 12 days treatment when compared to control untreated with percentage glycaemia change of 50.1,56.8, 52.8,
69.9 and 59.6 . Also two doses of n-butanol, 100 and 200 mg/kg fraction was administered to the diabetic rats.
The dose of 100 mg/kg, there was a significant decrease (p<0.05) after 7 and 12 days of treatment when
compared to untreated control. As regard the dose of 200 mg/kg, there was a significant decrease ( p<0.05) at 3,
5 ,7,9 and 12 days of treatment when compared to control untreated with percentage glycaemic change of
20.7,35.3,52.3, 44.2 and 40.9 respectively. The preliminary phytochemical screening revealed the presences of
saponin, flavonoid, tannin and alkaloid. The median lethal dose (LD50) in mice was calculated to be 471.2
mg/kg bodyweight. This result suggests that the Ethylacetate and n-butanol fractions of leaves methanolic extract
of Acacia nilotica possess antidiabetic effects on alloxan - induced diabetic Wistar rats.
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Keywords
Anti-diabetic, Alloxan, Ethylacetate, n-butanol Acacia nilotica
Citation
Tanko Y, Muhammad A, Elaigwu J, Mohammed K A, Jimoh A, Sada N M, Dallatu M K, Yerima M, Mohammed A. Anti-diabetic effects of ethyl acetate and n-butanol fractions of Acacia nilotica leaves methanolic extract on alloxan-induced diabetic wistar rats. Journal of Applied Pharmaceutical Science. 2013 Nov; 3(11): 89-93.