Stability indicating chromatographic techniques for the determination of pipoxolan HCl.
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Date
2013-10
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Abstract
Two simple, accurate and sensitive methods were developed for the determination of pipoxolan HCl in presence
of its degradation product. HPLC method (A), performed on C18 column using acetonitrile: 1mM ammonium
acetate (80:20 v/v) as a mobile phase with a flow rate of 1.8ml/min. Detection was performed at 210 nm. TLCdensitometric
method (B), using silica gel 60 F254 plates; the optimized mobile phase was chloroform: toluene:
methanol: 10% ammonia (6:5:3:0.1 v/v). Quantitatively the spots were scanned densitometrically at 210 nm.
Linearity ranges were 1 – 10 μg/ml for method A and 2-20 μg/band for method B, with mean percentage
recoveries 99.38±0.672% and 99.32±0.97% for methods A and B, respectively. The proposed methods were
found to be specific for pipoxolan HCl in presence of up to 90% of its degradation product. Statistical
comparison between the results obtained by these methods and the manufacturer’s method was done,and it was
found that there was no significant differences between them.
Description
Keywords
Pipoxolan HCl, HPLC, TLCdensitometric, Stability
Citation
Zaazaa Hala E, Mohamed Afaf O, Abdelkawy Mohamed, Hawwam Maha A. Stability indicating chromatographic techniques for the determination of pipoxolan HCl. Journal of Applied Pharmaceutical Science. 2013 Oct; 3(10): 66-73.