Characterization and antimicrobial activity of Amycolatopsis strains isolated from Thai soils.
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Date
2011-10
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Abstract
The isolation and screening of antimicrobial activity of 3 actinomycete strains isolated from soil samples
collected in Chaiyaphum, Nan and Phatthalung provinces, Thailand were carried out. Strains S39-7, KC19-1 and
K57-1 were belonged to the genus Amycolatopsis based on their phenotypic and chemotaxonomic characteristics.
On the basis of 16S rRNA gene sequence analysis, strain S39-7 was closely related to Amycolatopsis albidoflavus
KCTC 9471T (99.2%). Strains KC19-1 and K57-1 were closely related to A. kentuckyensis NRRL B-24129T
with 99.3 and 99.2% similarity, respectively. All of them contained meso-diaminopimelic acid (DAP) in cell wall
peptidoglycan and had MK-9 (H4) as a major menaquinones. The DNA G+C contents of the strains ranged from
67.2 to 73.4 mol%. On secondary screening of antimicrobial activity, the ethyl acetate extract of the
fermentation products of strain S39-7 was active against Staphylococcus aureus ATCC 6538, Bacillus subtilis
ATCC 6633, Escherichia coli ATCC 25922, Kocuria rhizophila ATCC 9341, and Pseudomonas aeruginosa
ATCC 27853 while strain KC 19-1 was active against only S. aureus ATCC 6538. Strain K 57-1 was active
against E. coli ATCC 25922 and K. rhizophila ATCC 9341. In addition strain S39-7 could inhibit against
methicillin resistant (MRSA) S. aureus 266.
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Keywords
Actinomycetes, Amycolatopsis, antimicrobial activity, soil
Citation
Sripairoj Piyapat, Suwanborirux Khanit, Tanasupawat Somboon. Characterization and antimicrobial activity of Amycolatopsis strains isolated from Thai soils. Journal of Applied Pharmaceutical Science. 2013 Oct; 3(10): 11-16.