New approach for measuring antioxidant activity via graphite sensor.
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Date
2013-09
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Abstract
An ion selective membrane sensor from dioctyl phthalate as a plasticizer in a polymeric matrix of polyvinyl
chloride (PVC) and β-cyclodextrin as an ionophore was constructed and evaluated according to IUPAC
recommendations. Linear Nernestian response of 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH•) within the
concentration ranges of 10−6 to 10−2 mol L−1 was obtained with average recovery 99.87±0.617. Nernstian slope of
58.5 mV/decade with excellent selectivity over the pH range of 3–8 was observed. The suggested method was
standardized using butylated hydroxyl anisole (BHA). The 50% radical scavenging activity (IC50) determined by
the proposed sensor correlated well with that of the common colorimetric method based on scavenging of DPPH•.
An algorithm implemented in Microsoft Visual Basic® 6.0 was used for calculating (IC50) values which are 7.38
μg/ml ± 0.35, 89.98 μg/ml ± 0.45 and 1.45 mg/ml± 1.50 for BHA, paracetamol and dipyridamole, respectively.
The proposed sensor represents a simple and reproducible tool for measuring DPPH• scavenging activity of
paracetamol and dipyridamole in bulk powder, pharmaceutical formulations and simulated intestinal fluid (SIF)
without sophisticated separation techniques.
Description
Keywords
Sensor, antioxidant, paracetamol, dipyridamole
Citation
El-Kosasy M, Hussien L A, Abdel-Rahman M H. New approach for measuring antioxidant activity via graphite sensor. Journal of Applied Pharmaceutical Science. 2013 Sept; 3(9): 71-77.