Optimized Ultrasonic-Assisted Extraction of Polysaccharides from Chinese Traditional Medicinal Plant Paris fargesii Using Orthogonal Design.
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Date
2013-05
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Abstract
Paris fargesii is a famous Chinese traditional medicinal plant used as antipyretic, antidotal, antiphlogistic and
analgesic. This research aimed at the determination of the total polysaccharides contents in P. fargesii, which
were collected from Baoxing country of Sichuan Province in order to get the optimized extraction technology.
Ultrasonic method was adopted in the extraction of total polysaccharides from P. fargesii. Through single factor
experiment and orthogonal test, the effects of ultrasonic extraction conditions on the total polysaccharides
extraction were measured, including solid-liquid ratio, ultrasonic temperature, ultrasonic power and ultrasonic
time. Then colorimetric method was applied for the assaying. The optimized extraction conditions of total
polysaccharides from P. fargesii were as follows: ultrasonic power: 600w, solid-liquid ratio: 1:20, ultrasonic
extraction time: 1.5h and ultrasonic temperature: 90℃. Under the optimum parameters, the total polysaccharides
extraction efficiency was 0.0715%.
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Keywords
Paris fargesii, total polysaccharides, ultrasonicassisted extraction, orthogonal design
Citation
Xiang Gang, Wang Dan, Zou Gui-Hua, Huang Xing, Tian Meng-Liang, Gao Feng. Optimized Ultrasonic-Assisted Extraction of Polysaccharides from Chinese Traditional Medicinal Plant Paris fargesii Using Orthogonal Design. Journal of Applied Pharmaceutical Science. 2013 May; 3(5): 110-113.