Phytochemical screening and invitro determination of antioxidant potential of methanolic extract of streospermum chelonoides.
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Date
2013-03
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Abstract
Plant derived phytochemicals consisting of phenols and flavonoids possess antioxidant properties, eventually
renders a lucrative tool to scavenge reactive oxygen species (ROS). Current study evaluates the preliminary
phytochemical screening and antioxidant activity of methanolic extract of bark and leaf of Stereospermum
chelonoides. Thus, various in vitro assay strategies were implemented to evaluate antioxidant potential of
Stereospermum chelonoides, using DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging assay, ferric reducing
antioxidant power (FRAP), total antioxidant capacity, determination of total phenol and flavonoid contents.
Preliminary phytochemical study revealed the presence of alkaloid, carbohydrate, glycoside, flavonoid, steroid
and tannin. In DPPH radical scavenging assay, the fraction showed significant antioxidant activities in the assay
compared to the reference ascorbic acid in a dose dependent manner. The IC50 value of the crude methanol
extract of bark and leaf was 53.99±3.25 μg/mL and 84.73±4.02 μg/mL, respectively, whereas IC50 value for the
reference ascorbic acid was 14.56±0.24 μg/mL. Moreover, profound total antioxidant activity was observed for
bark (309.88±1.03 mg/g equivalent to ascorbic acid) as well as leaf (147.09±1.79 mg/g equivalent to ascorbic
acid) at 200 μg/mL extract concentration. Furthermore, extract showed good reducing power capability in both
bark and leaf fraction. Total phenol content for the bark was 574.82 mg/g equivalent to gallic acid and for leaf
was 189.86 mg/g. For bark, the total flavonoid content was found 55.82 mg/g equivalent to quercetin and for leaf
it was 49.44 mg/g.
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Keywords
Stereospermum chelonoides, antioxidant potential, DPPH, ferric reducing antioxidant power (FRAP)
Citation
Shanta Meena Afroze, Ahmed Taksim, Nasir Uddin Mohammad, Majumder Senjuti, Hossain Md Solayman, Rana Md Sohel. Phytochemical screening and invitro determination of antioxidant potential of methanolic extract of streospermum chelonoides. Journal of Applied Pharmaceutical Science. 2013 Mar; 3(3): 117-121.