Formulation and in vitro/in vivo Evaluation of Zidovudine Contained in Solidified Reverse Micellar Delivery System of Immune Compromised Rats.
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Date
2013-02
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Abstract
Aim of the study was to study the in vitro and in vivo evaluation and correlation of zidovudine (AZT) loaded
solidified reverse micellar microparticles (SRMMs). The SRMMs composed of goat fat and Phospholipon® 90H
in various ratios (1:1, 2:1, 3:1 and 2:3) were prepared by melt dispersion method. AZT (1 %w/w, 2 %w/w, 3
%w/w and 5 %w/w) were incorporated into the SRMMs and preliminary analysis of the preparations on their
stability were done visually. The 1:1 formulation was evaluated for the particle size, percentage yield and in vitro
studies which was done using SGF and SIF. The in vivo study was done using Wistar albino rats and the in vitroin
vivo correlation (IVIVC) was determined by plotting a graph of the fraction of drug absorbed in vivo versus the
fraction of drug released in vitro. The yield of the goat fat extraction was 58 %. The particle size and yield of the
solid lipid microparticle (SLM) containing 1 %w/w of AZT were 5.10 ± 0.10m and 86.3 ± 4.70% respectively.
The fraction of drugs absorbed in vivo were 0.102 μg, 0.114 μg, 0.115 μg, 0.134 μg and 0.123 μg for 1 h, 3 h, 5
h, 8 h and 12 h respectively. A 1:1 ratio of goat fat and Phospholipon® 90H with a high value of correlation
coefficient (r2 = 0.909) suggested good level-A correlation between the in vitro-in vivo data of the SLM obtained
in the study.
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Keywords
solidified reverse micellar microparticle (SRMM),, lipid matrix, in vitro-in vivo correlation, Phospholipon® 90H, immune-compromised
Citation
Uronnachi Emmanuel M, Ogbonna John D N, Kenechukwu Franklin C, Attama Anthony A, Okore Vincent C. Formulation and in vitro/in vivo Evaluation of Zidovudine Contained in Solidified Reverse Micellar Delivery System of Immune Compromised Rats. Journal of Applied Pharmaceutical Science. 2013 Feb; 3(2): 31-35.