Phytochemical Analysis and Antiviral Potential of Aqueous Leaf Extract of Psidium guajava Against Newcastle Disease Virus in ovo.
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Date
2012-10
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Abstract
Leaf extract of Psidium guajava were subjected to phytochemical screening and in ovo antiviral
assay against Newcastle Disease Virus (NDV). Phytochemical analysis revealed the presence of
pharmacologically active and nutritionally relevant compounds. Nine-day-old embryonated
chicken eggs were divided into ten groups of fives and received various treatments. Groups 1-6
received 100EID50/0.1ml NDV pre-treated with P. guajava leaf extracts at final concentrations of
250, 200, 100, 50, 25 and 10mg/ml in that order. Controls were included. Embryo survival was
observed daily. Allantoic fluid from treated eggs and serum from hatched chicks were collected
for spot hemagglutination (HA) and hemagglutination inhibition (HI) tests to detect NDV in the
eggs and antibodies against NDV in the hatched chicks respectively. Results showed that embryo
survival was higher with higher extract concentrations.. Just as increase in extract concentration
was directly proportional to virus death and inversely proportional to production of antibody
against NDV in hatched chicks. The current findings have clearly demonstrated that P. guajava
leaf extract has nutritional value as well as great antiviral potential against NDV in ovo. In vivo
trials are needed to validate the use of the tree in controlling Newcastle disease in chickens.
Description
Keywords
Phytochemical Screening, Newcastle Disease Virus, Psidium guajava, In ovo
Citation
Chollom S C, Agada G O A, Bot D Y, Okolo M O, Dantong D D, Choji T P, Echeonwu B C, Bigwan E I, Lokason S, Banwat E. Phytochemical Analysis and Antiviral Potential of Aqueous Leaf Extract of Psidium guajava Against Newcastle Disease Virus in ovo. Journal of Applied Pharmaceutical Science. 2012 Oct; 2(10): 45-49.