Comparison between Microplate Spectrometry and LC/MS Chromato-graphy for Facile Pilot Pharmacokinetics and Biodistribution Studies of Doxorubicin-loaded Nanoparticle Drug Carriers.
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Date
2012-09
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Abstract
facile and reliable method to perform pilot pharmacokinetic (PK) and biodistribution studies is necessary
for expediting the overall development and clinical translation of novel nanoparticle drug carriers. In this
study, we compared two common analytical techniques, fluorescence spectrometry using a microplate reader
and liquid chromatography/mass spectrometry (LC/MS), demonstrating the quantification of a model
anticancer drug (doxorubicin: DOX) in its free drug and nanoparticle formulations in vivo. Drug-loaded
nanoparticle formulations were prepared from poly(ethylene glycol)-poly(aspartate) block copolymers,
which formed two model drug carriers with different particle stability, self-assembled polymer micelles
(DOX-micelles) and cross-linked nanoassemblies (DOX-CNAs). These three DOX formulations were
injected into tumor-bearing mice at a DOX equivalent concentration. DOX levels in liver, spleen, and tumors
were found to be comparable regardless of the analytical methods. LC/MS showed lower serum level than
spectrometry with a microplate reader, which is consistent with the fact that DOX metabolites are present
mainly in the serum.These results demonstrate that, in comparison to the LC/MS method, spectrometry using
a microplate reader would be a viable and more facile method to perform pilot PK and biodistribution studies
of various potential nanoparticle drug carriers using DOX as a model drug.
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Keywords
Drug Delivery, Polymer Micelles, Cross-linked Nanoassemblies, Doxorubicin, Pharmacokinetics, Biodistribution
Citation
Cao Pengxiao, Bae Younsoo. Comparison between Microplate Spectrometry and LC/MS Chromato-graphy for Facile Pilot Pharmacokinetics and Biodistribution Studies of Doxorubicin-loaded Nanoparticle Drug Carriers. Journal of Applied Pharmaceutical Science. 2012 Sept; 2(9): 1-9.