Kalanchoe thrysiflora Harv. and Kalanchoe marmorata Baker; DNA Profiling, biological guided fractionation of different extracts; isolation and identification of cytotoxic compounds.
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Date
2012-08
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Abstract
DNA profiling of two closely related ornamental plants belonging to family
crassulaceae viz. Kalanchoe thrysiflora Harv. and Kalanchoe marmorata Baker were performed to
establish genetic polymorphism. Biological guided fractionation of the two plant extracts to assess
their cytotoxicity, had led to the isolation of one steroidal and one triterpenoidal compound from
the most active dichloromethane fraction of Kalanchoe thrysiflora. The cytotoxicity of the
isolated compounds were evaluated against normal (HFB4) and cancer (MCF7) cells. Compound
1 (3-oxo-olean-12-ene) and compound 2 (β-sitosterol) showed similar cytotoxic activity on MCF7
at IC50 17.4 and 17.6 μg/ml respectively while on HFB4, the compounds revealed cytotoxic
activity at IC50 21.9 and 21.6 respectively.
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Keywords
Crassulaceae, cytotoxicity, DNA profiling, Kalanchoe thrysiflora, Kalanchoe marmorata
Citation
Singab Abdel Nasser, El-Ahmady Sherweit, Milad Rola, Saad Sally. Kalanchoe thrysiflora Harv. and Kalanchoe marmorata Baker; DNA Profiling, biological guided fractionation of different extracts; isolation and identification of cytotoxic compounds. Journal of Applied Pharmaceutical Science. 2012 Aug; 2(8): 215-220.