Comparitive Pharmacognostical and Phytochemical Evaluation of Brihat and Laghu Gokshur.
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Date
2012-08
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Abstract
Laghu Gokshur (Tribulus terrestris) and Brihat Gokshur (Pedalium murex) are well
known drugs used in Ayurveda as diuretics. These are classified under mishrak varga as
‘Dashmoola’ in Ayurveda and in chemotaxonomy as Saponin glycosides. The objective of the
work is to find out the diagnostic tool to identify the two varieties of Gokshur. The powder was
studied for macroscopic, microscopic and physicochemical parameters. For HPTLC Stationary
phase was Pre-coated silica gel GF 254 and mobile phase was Toluene: Ethyl acetate: Formic
acid (7:2:1 v/v/v). The plate was scanned and quantified at 254 nm for Diosgenin.Results shows
that microscopic characters like trichomes, stomata and crystals show some difference in the
two varieties while physicochemical parameters show difference in extractive values.
Phytochemical screening also shows similar findings. HPTLC analysis carried out using
Diosgenin as reference standard revealed the presence of steroidal Saponin “Diosgenin”.
Quantitative estimation for Saponins found marked variation in the two varieties, where Laghu
gokshur had 16% of Saponins while Brihat gokshur had 13% Saponins. The study can be used
as a diagnostic tool for identification of these two varieties of Gokshur.
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Keywords
Gokshur, Microscopy, Physicochemical parameters, HPTLC fingerprint
Citation
Renuka, Khanpara Komal, Shukla V J, Prajapati P K, Harisha C R, Pandiya Preeti. Comparitive Pharmacognostical and Phytochemical Evaluation of Brihat and Laghu Gokshur. Journal of Applied Pharmaceutical Science. 2012 Aug; 2(8): 124-127.