Standardization of Ethanolic Extract of Cucurbita Maxima Seed.
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Date
2012-08
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Abstract
Standardization is an important step for the establishment of a consistent biological
activity, a consistent chemical profile, or simply a quality assurance program for production and
manufacturing of herbal drugs preparation of any herbal formulation identification, evaluation
and standardization is rudimentary identification involves the morphology, microscopy
parameter of plants, evaluation and standardization of herbal drugs includes physical, chemical
and biological parameters. These parameters are crucial for preparation of accurate and potent
formulation. The present communication attempts to investigate pharmacognostical and
phytochemical details of Cucurbita maxima, (Cucurbitaceae). The Preliminary phytochemical
analysis revealed presence of carbohydrates, alkaloids, flavonoids, saponins, proteins and
amino acids in alcoholic extract. HPTLC studies reveal that alcoholic extract gives 3 spots and
alcoholic extract depicts 5 spots on the TLC plate in Butanol: acetic: water solvent system with
Ninhydrin as spraying agent and 3 spots with vanillin as spraying agent and with butanol:
phenol: water (6:1:1) 4 spots were seen with Ninhydrin as spraying agent and 2 spots were seen
with vanillin as spraying agents. The GC/MS of pet ether methyl ester showed number of
peaks. Out of which 3 highest peaks in descending order were taken into consideration.
OSAZONE were formed which showed needle shaped crystals of glucaosazone. The study
revealed specific identities for which may play a key role in identification of plant and can be
useful in standardization of the herbal drugs.
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Keywords
Cucurbita maxima, ethanolic extract, Cucurbita maxima seed, Cucurbitacins
Citation
Bajpai Richa, Jain Nidhi, Pathak A K. Standardization of Ethanolic Extract of Cucurbita Maxima Seed. Journal of Applied Pharmaceutical Science. 2012 Aug; 2(8): 92-95.