Antimicrobial Activity of Crude Extracts of Endophytic Fungi Isolated from Medicinal Plant Trichilia elegans A. Juss.
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Date
2012-08
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Abstract
Endophytes are microorganisms which inhabit inside plants. These microorganisms
are identified as being fungi or bacteria and can be useful for prospectionof bioactive
compounds that may have medical and pharmaceutical applications.Trichilia elegans
(Meliaceae) is a native tree in Brazil. Preparations using leaves, seeds, bark and roots and some
members of the Trichilia genus are used in Brazilian popular medicine.The aim of the present
work was to investigate biotechnological potential of fungal endophytes (Cordyceps
memorabilis, Phomopsis longicolla, Dothideomycete sp. and one non-indentified) isolated from
T. elegans, that have been assayed against five pathogenic bacteria. The fungi were incubated in
Potato Dextrose and the secondary metabolites was extracted from fermentation medium with
ethyl acetate, also was used directly extraction with methanolfrom mycelium. The extraction by
ethyl acetate from C. memorabilis inhibited growing of Enterococus hirae, Micrococcus luteus
and Escherichia coli. Fungal Phomopsis sp. inhibited M. luteus, E. hirae and Salmonella typhi,
Dothideomycetes sp. and G8-25 inhibited M. luteus and E. hirae. Noextract by ethyl acetate
inhibited Staphylococcus aureus and also no extract obtained by methanol inhibited the growing
of tested bacteria. The present study helped justified the traditional use of T. elegans against
human pathogenic bacteria.
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Keywords
Trichilia elegans, fungal endophytes, bioative compounds, pathogenic bacteria
Citation
Rhoden S A, Garcia A, Bongiorno V A, Azevedo J L, Pamphile J A. Antimicrobial Activity of Crude Extracts of Endophytic Fungi Isolated from Medicinal Plant Trichilia elegans A. Juss. Journal of Applied Pharmaceutical Science. 2012 Aug; 2(8): 57-59.