Method Development and Validation of Levosalbutamol in Pure and Tablet Dosage Form by RP-HPLC.
Loading...
Date
2012-06
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
A simple, rapid and accurate RP-HPLC method was developed for the determination of
levosalbutamol in pure and tablet dosage form by RP-HPLC method using C18 BDS column
(Phenomenex, 250 x 4.6 mm, 5 μm) in isocratic mode. The mobile phase consisted of
Acetonitrile and buffer in the ratio of 20:80 (v/v) was used and maintain the pH 3. The flow rate
was maintained at 1 mL/min and the injection volume was 20 μL . Detection wavelength with
UV detector at 276 nm and run time was kept 10 min. The retention time of levosalbutamol was
5.4 min. The method was linear over the concentration range 7-12 μg/ml. The recovery was
found to be 100.44± 0.27%. The validation of method was carried out utilizing ICH-guidelines.
The described HPLC method was successfully employed for the analysis of pharmaceutical
formulations.
Description
Keywords
HPLC method, Levosalbutamol, Method development
Citation
Nyola Narendra, Jeyabalan Govinda Samy, Yadav Garima, Yadav Rajesh, Gupta Subash, Khalilullah Habibullah. Method Development and Validation of Levosalbutamol in Pure and Tablet Dosage Form by RP-HPLC. Journal of Applied Pharmaceutical Science. 2012 June; 2(6): 155-158.