Determination of furosemide and zonisamide as a drug substance and in dosage form by ion pair - reversed phase liquid chromatographic technique.
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Date
2012-05
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Abstract
An isocratic, selective, and accurate Ion pair - reversed phase liquid chromatographic
method of analysis of Furosemide and Zonisamide both as a bulk drug and formulations was
developed and validated. An ODS chromatographic column (250mmx4.6mm, 5μm) was used for
the separation. The mobile phase consisted of a mixture of methanol and Tetrabutyl ammonium
hydrogen sulphate (TBHS) 0.035 molar aqueous solution, pH adjusted to 6.0 using 1 N sodium
hydroxide solution. The composition of TBHS with methanol used for Furosemide was
40:60(v/v) and that for Zonisamide was 50:50(v/v) delivered at a flow rate of 1.0ml/min and
detection at wave length 240 nm. The developed method was validated in terms of selectivity,
Linearity, limit of quantitation, precision, accuracy and solution stability. The proposed LC
method achieved satisfactory resolution between Furosemide and 4-Chloro-5-sulphamoyl
salicylic acid (CSSA), Zonisamide and CSSA an intermediate product possibly present in
Furosemide and Zonisamide. The method can be employed as stability indicating method for
both the drugs furosemide and zonisamide and its dosage form.
Description
Keywords
Ion pair, Reversed phase, Zonisamide, Furosemide
Citation
Rao B Udaykumar, Nikalje Anna Pratima. Determination of furosemide and zonisamide as a drug substance and in dosage form by ion pair - reversed phase liquid chromatographic technique. Journal of Applied Pharmaceutical Science. 2012 May; 2(5): 94-99.