Evaluation of dimethylthiazol diphenyl tetrazolium bromide and propidium iodide inclusion assays for the evaluation of cell viability by flow cytometry.
Loading...
Date
2012-05
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The natural products are widely used in many fields as pharmaceutical, flavor,
industrial, and additive compounds. In many cases, there is a need to evaluate the cytotoxic
effect of these products and to determine the non cytotoxic concentration at which they can be
used in a safe manner. The objective of the present study was to compare two methods of
evaluation the cytotoxic effect of a plant extract. The MTT assay and the PI dye-inclusion assay
were used to carry out the experiments. The results were then evaluated by calorimetric and flow
cytometry assays. The obtained results showed that a clear difference between the two methods
and the cell death using the MTT assay seem to be overestimated. To better evaluate the
cytotoxic effect of natural product, we need to carry out several in vitro and in vivo tests before
the judgment that a compound is safe to use and at which concentration.
Description
Keywords
MTT assay, Cytotoxicity, Propodium Iodide, Flow cytometry, natural extract
Citation
Abid Nabil Ben Salem, Rouis Zyed, Nefzi Faten, Souelah Nidhal, Aouni Mahjoub. Evaluation of dimethylthiazol diphenyl tetrazolium bromide and propidium iodide inclusion assays for the evaluation of cell viability by flow cytometry. Journal of Applied Pharmaceutical Science. 2012 May; 2(5): 10-14.