Q-Absorbance Ratio Spectrophotometric Method for the Simultaneous Estimation of Rifampicin and Piperine in their Combined Capsule Dosage.
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Date
2012-04
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Abstract
The present manuscript describe simple, sensitive, rapid, accurate, precise and
economical Q-absorbance ratio method for the simultaneous determination of Rifampicin and
Piperine in combined capsule dosage form. Absorbance ratio method uses the ratio of
absorbances at two selected wavelengths, one which is an isoabsorptive point and other being the
λ-max of one of the two components. Rifampicin and Piperine show an isoabsorptive point at
387 nm in methanol. The second wavelength used is 337 nm, which is the λ-max of Piperine in
methanol. The linearity was obtained in the concentration range of 5-40 μg/ml for Rifampicin
and 2-20 μg/ml for Piperine. The concentrations of the drugs were determined by using ratio of
absorbances at isoabsorptive point and at the λ-max of Rifampicin. The method was successfully
applied to pharmaceutical dosage form because no interference from the capsule excipients was
found. The results of analysis have been validated statistically and by recovery studies.
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Keywords
Rifampicin, Piperine, absorbance ratio method, isoabsorptive point, validation, simultaneous
Citation
Khamar Jenil C, Patel Satish A. Q-Absorbance Ratio Spectrophotometric Method for the Simultaneous Estimation of Rifampicin and Piperine in their Combined Capsule Dosage. Journal of Applied Pharmaceutical Science. 2012 Apr; 2(4): 137-141.