Eco friendly spectrophotometric method for quantitative estimation of lomefloxacin using hydrotropic approach.
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Date
2012-04
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Abstract
The present work describes a novel, accurate, sensitive and economic safe
spectrophotometric method was developed by application of hydrotropy, using 8 M Urea
solution as hydrotropic solubilizing agent, for the quantitative determination of poorly watersoluble
lomefloxacin HCl in tablet dosage form. There were more than 43 times enhancements
in the solubility of lomefloxacin HCl increases in hydrotropic solution as compared to
solubilities in distilled water. Lomefloxacin HCl shows maximum absorbance at 281 nm. Urea
and other tablets excipents did not show any absorbance above 230 nm, and thus no interference
in the estimation was seen. Lomefloxacin HCl was obeyed Beer,s law in the concentration range
of 5 to 25μg/ml (r2= 0.9998) in hydrotropic solvent with mean recovery ranging from
98.03±0.65 to 98.59±0.32%. Proposed method is new, simple, economic, safe, rapid, accurate
and reproducible. The developed methods were validated according to ICH guidelines and
values of accuracy, precision and other statistical analysis were found to be in good accordance
with the prescribed values. The method can be used for routine analysis in both research
laboratories, and pharmaceutical and chemical industries to analyze the drugs without the use of
organic solvents thus make the environment eco-friendly.
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Keywords
Lomefloxacin HCl, Urea, Spectrophotometry, Eco-friendly, Hydrotropic
Citation
Jain Ruchi, Jain Vaibhav, Jain Nilesh, Jain Deepak Kumar, Jain Surendra. Eco friendly spectrophotometric method for quantitative estimation of lomefloxacin using hydrotropic approach. Journal of Applied Pharmaceutical Science. 2012 Apr; 2(4): 111-114.