High Performance Liquid Chromatography (HPLC) Method Development and Validation Indicating Assay for Ciprofloxacin Hydrochloride.
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Date
2011-10
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Abstract
A new simple, rapid, selective, precise and accurate isocratic reverse phase high
performance liquid chromatography assay has been developed for the estimation of Ciprofloxacin
Hydrochloride in tablet formulation. The separation was achieved by using C-18 column
(LichroCART® 125x4mm, 5μm) coupled with a guard column of silica in mobile phase methanol:
buffer (0.025M Orthophosphoric acid with the pH adjusted to 3.0±0.1 with triethylamine)
(40:60v/v). The flow rate was 2.0ml/min and the drug was detected using UV detector at the
wavelength of 278nm. The retention time was within 1.753 – 1.757 minutes. The method was
validated as per ICH guidelines. The proposed method was found to be accurate, repeatability and
consistent. It was successfully applied for the analysis of the drug in marketed formulation and
could be effectively used for the routine analysis of formulation containing the drug without any
alteration in the chromatography conditions.
Description
Keywords
Ciprofloxacin, HPLC, Liquid Chromatography
Citation
Ali Sani A, Mmuo Chijioke C, Abdulraheem Rafat O, Abdulkareem Sikirat S, Alemika Emmanuel T, Sani Musa A, Ilyas Mohammed. High Performance Liquid Chromatography (HPLC) Method Development and Validation Indicating Assay for Ciprofloxacin Hydrochloride. Journal of Applied Pharmaceutical Science. 2011 Oct; 1(8): 239-243.