Studies on extended beta lactamase producing, biofilm forming clinical bacterial pathogens and its invitro inhibition by Actinobacterial extracts.
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Date
2011-10
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Abstract
At present scenario, the extended spectrum beta lactamase (ESBL) producing
bacterial pathogen causes various life threatening infections especially by the members of the
family Enterobacteriaceae in hospital settings. In order to study the prevalence of ESBLs in
Kanchipuram hospital, the bacterial strains were isolated from patients having Urinary tract
infections (UTI), diabetic foot ulcer, pregnancy women’s, surgical wound infections, deep
wounds, and genitourinary tract problems. Totally 40 bacterial isolates were recovered from 30
samples and the isolates were identified as Escherichia coli (45%), Pseudomonas sp, (25%) and
Klebsiella sp (30%). The ESBL production was confirmed with third generation cephalosporins
(cetixime, cephoxitin, ceftazidime, cetepime, ceftriaxone, ceftazidime/clavulanic acid) using the
Kirby- bauer disc diffusion method and also by double disc diffusion method. The highest
ESBL production was found among E. coli (42%), followed by Pseudomonas sp. (25%) and
Klebsiella sp (20%). All the ESBL producer were tested for biofilm formation by tube method
in which E.coli (43%) was found to be the good biofilm producer followed by the Klebsiella sp
(31%) and Pseudomonas sp (25%). An attempt was also made to study the in-vitro inhibition of
biofilm forming ESBL pathogens by actinobacterial extracts by disc diffusion method. Of the
five actinobacterial extracts tested, extracts produced from the strain MA7 inhibited (8-12 mm
zone of inhibition) all the biofilm forming ESBL pathogens. Further purification and
characterisation of active compound from actinobacterial strain MA7 is in progress.
Description
Keywords
bacterial pathogens, ESBL production, biofilm, actinobacterial extracts
Citation
Hemachandran K, Bharathi S, Radhakrishnan M, Balagurunathan R. Studies on extended beta lactamase producing, biofilm forming clinical bacterial pathogens and its invitro inhibition by Actinobacterial extracts. Journal of Applied Pharmaceutical Science. 2011 Oct; 1(8): 210-213.