Enhancement of camptothecin production in Camptotheca acuminata hairy roots by overexpressing ORCA3 gene.
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Date
2011-10
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Abstract
Being a good anti-tumor drug, camptothecin (CPT) is a kind of modified monoterpene
indole alkaloid firstly isolated from the deciduous Chinese happy tree with rapidly increasing
clinical demand. Due to the great importance and low content of this compound, it is very
important to improve CPT production by modern biotechnology. ORCA3 is a jasmonateresponsive
APETALA2-domain transcript factor isolated from Catharanthus roseus, with strong
ability to up-regulate expression of serveral key genes involved in TIA biosynthetic pathway. To
investigate physiological function of ORCA3 gene in Camptotheca acuminata, the ORCA3 gene
was transformed using Agrobacterium-mediated gene transfer technology. PCR analysis
confirmed that the ORCA3 gene was integrated into the plant genome. HPLC showed that
overexpression of ORCA3 in transgenic hairy root lines can effectively enhance the production of
camptothecin with 1.5-fold compared with the control (1.12 mg/g dw). The results revealed that
ORCA3 is an effective regulatory gene for improving metabolic flux in camptothecin biosynthetic
pathway at the first time.
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Keywords
Camptotheca acuminata, ORCA3, camptothecin, transformation, hairy root cultures
Citation
Ni Xiaoning, Wen Shiqiu, Wang Wei, Wang Xiaoyun, Xu Hui, Kai Guoyin. Enhancement of camptothecin production in Camptotheca acuminata hairy roots by overexpressing ORCA3 gene. Journal of Applied Pharmaceutical Science. 2011 Oct; 1(8): 85-88.