Micropropagation and shoot regeneration of Cistus creticus ssp. Creticus.
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Date
2011-10
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Abstract
Cistus creticus L. ssp. creticus is a medicinal aromatic shrub native in Crete (Greece).
The protocol described in this paper provides optimal levels of growth regulators required to
obtain high regeneration rates of Cistus in vitro. Micropropagation has been achieved through
rapid proliferation of shoot-tips on Murashige and Skoog (MS) basal medium supplemented with
0.2 mg l-1 6-benzylaminopurine (BAP). After four weeks shoots were transferred to MS medium
without growth regulators for further development and rooting. The highest percentage of
regenerated shoots was obtained with 0.1 mg l-1 TDZ and 0.1 mg l-1 NAA after 4 weeks.
Elongation and rooting was readily achieved when multiple shoots more than 1 cm in length were
singled out and cultured on the MS medium without growth regulators. The plantlets were
successfully adapted and grew vigorously in greenhouse conditions. This is the first report of
shoot regeneration in the genus Cistus. The regeneration protocol developed in this study provides
a basis for further investigation of the medicinally active constituents of this elite medicinal plant.
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Keywords
α-naphthaleneacetic acid (NAA), cretan ladano, shoot regeneration, thidiazuron (TDZ).
Citation
Madesis Panagiotis, Konstantinidou Elpiniki, Tsaftaris Athanasios, Nianiou-Obeidat Irini. Micropropagation and shoot regeneration of Cistus creticus ssp. Creticus. Journal of Applied Pharmaceutical Science. 2011 Oct; 1(8): 54-58.