In-vitro interaction of verapamil hydrochloride with magnesium sulphate (anhydrous) and its influence on protein binding of verapamil hydrochloride.
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Date
2011-08
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Abstract
The interaction between Verapamil Hydrochloride and Magnesium Sulphate
(anhydrous) has been studied in an aqueous system at pH 7.4 and 2.4. From spectrophotometric
study, it has been found that Verapamil Hydrochloride form 1:1 complex with Magnesium
Sulphate (anhydrous). Spectral studies helps to detect the initial complexation between drug and
metal. Job’s plot at 7.4 and 2.4 provides same type of information. The Ardon’s
spectrophotometric method confirmed the 1:1 complexation and the value of stability constants
was calculated using Ardon’s plot. An in vitro study of protein binding of Verapamil
Hydrochloride and their 1:1 mixture with Magnesium Sulphate (anhydrous) has been conducted
by equilibrium dialysis method at (37 ± 0.5)0C and at pH 7.4. The Scatchard plots were prepared
to reveal the number of binding sites and the affinity for protein binding. It has been found that
interaction of the drug with Magnesium Sulphate (anhydrous) results into increasing the affinity
and increasing the protein binding of Verapamil Hydrochloride.
Description
Keywords
Verapamil Hydrochloride, Protein binding, Equilibrium dialysis, Scatchard plot, Magnesium Sulphate (anhydrous), Ardon’s method
Citation
Das Joysree, Khan Irfan Newaz, Siraji Fouzia, Sharif Syeda Ridita, Ajrin Marzina. In-vitro interaction of verapamil hydrochloride with magnesium sulphate (anhydrous) and its influence on protein binding of verapamil hydrochloride. Journal of Applied Pharmaceutical Science. 2011 Aug; 1(6): 122-126.