Quantitative analysis of valsartan in tablets formulations by High Performance Thin-Layer Chromatography.
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Date
2011-06
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Abstract
A new, simple, accurate, and precise high-performance thin-layer chromatographic
(HPTLC) method has been established for quantitative analysis of valsartan in tablet formulations.
Standard and sample solutions of valsartan were applied to precoated silica gel G 60 F254 HPTLC
plates and the plates were developed with chloroform: acetonitrile: toluene: glacial acetic acid, in
the ratio 1:8:1:0.1 (v/v) (v/v), as mobile phase. UV detection was performed at 254 nm. The
retention factors of valsartan was 0.65. The calibration plot for Valsartan standard was linear with
r =0.9999, slope = 5.328 and intercept=356.9. The limit of detection and limit of quantitation of
Valsartan were found to be 5and 16 ng per spot respectively. The percentage recovery was found
to be 99.37% for Valsartan. The method showed good repeatability and recovery with relative
standard deviation less than 2. Method was validated in accordance with the requirements of ICH
guidelines and was shown to be suitable for purpose. The method is selective and specific can be
used for determination of the routine analysis of valsartan in tablets. Tablet excipients did not
interfere with the chromatography.
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Keywords
Valsartan, HPTLC method, Method validation, mobile phase
Citation
Parambi Della Grace Thomas, Mathew Molly, Ganesan V. Quantitative analysis of valsartan in tablets formulations by High Performance Thin-Layer Chromatography. Journal of Applied Pharmaceutical Science. 2011 Jun; 1(4): 76-78.