In vitro propagation of a rare medicinal fern of Western Ghats – Diplazium esculentum (Reytz.).
Loading...
Date
2011-11
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Present study aimed for in vitro culture of circinate part of young leaves of D. esculentum which is amongst the leafy vegetables consumed as vegetable by Paniya and Chetti tribes of Western Ghats. The circinate part of young leaves (crosiers), excised before the beginning of foliar expansion, was inoculated on half strength Murashige and Skoog (MS) medium supplemented with auxins indole-3-butyric acid (IBA) or α-napthalene acetic acid (NAA) or 2,4-Dichlorophenoxyacetic acid (2,4-D) and cytokinin 6- benzylaminopurine (BA) in a range 0.5 to 2.5 mg L-1. Combinations of different concentrations of 2,4 D + BA, IBA + BA as well as of NAA+ BA were also tested in half strength MS medium with 3% sucrose and with pH 5.8. The best morphogenic response was obtained with half strength MS medium supplemented with 2,4-D 0.5 mg L-1 and BA 2.5 mg L-1, 3% sucrose, at pH 5.8. For rooting of the microshoots, half strength MS medium supplemented with 2,4-D ( 2 and 1 mg L-1 ) exhibited best results. Present study reports the successful in vitro culturing of D. esculentum.
Description
Keywords
Diplazium esculentum, Dryopteridaceae, MS medium, In vitro shooting
Citation
Nair Archana G, Pradeesh S, Nikhila G S, Sangeetha G, Mini I, Swapna T S. In vitro propagation of a rare medicinal fern of Western Ghats – Diplazium esculentum (Reytz.) Indian Journal of Experimental Biology. 2013 Nov; 51(11): 919-923.