Assessment of somatic embryogenesis potency in Indian soybean [Glycine max (L.) Merr.] cultivars.
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Date
2013-10
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Abstract
Majority of the Indian soybean cultivars are recalcitrant to tissue culture regeneration. The present communication reports the development of somatic embryogenesis in a liquid culture medium from immature cotyledons of G. max. Following induction with 2,4-dichlorophenoxyacetic acid (2,4-D) or naphthalene acetic acid (NAA), the number of somatic embryos and percentage of explants that responded were higher with 45.24 µM 2,4-D. The proliferation of somatic embryos for three successive cycles was achieved in 22.62 µM 2,4-D. Histodifferentiation of somatic embryos under NAA (10.74 µM) indicated that better embryo development and maturation was achieved without any growth regulator. The amino acids such as L-glutamine favoured the somatic embryo induction and histodifferentiation at 20 and 30 mM respectively, where as L-asparagine at 10 mM concentration enhanced the somatic embryo proliferation. In addition, somatic embryos that were desiccated (air-drying method) for 5 days showed better germination (40.88%). The Indian soybean cultivars also showed strict genotypic influence and cv. Pusa 16 was emerged as a best responding cultivar for somatic embryo induction with 74.42% of response.
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Keywords
Amino acids, Genotype, Immature cotyledon, Somatic embryogenesis, Soybean
Citation
Mariashibu Thankaraj Salammal, Subramanyam Kondeti, Arun Muthukrishnan, Theboral Jeevaraj, Rajesh Manoharan, Rengan Sampath Kasthuri, Chakravarthy Rajan, Manickavasagam Markandan, Ganapathi Andy. Assessment of somatic embryogenesis potency in Indian soybean [Glycine max (L.) Merr.] cultivars. Indian Journal of Experimental Biology. 2013 Oct; 51(10): 849-859.