Simultaneous detection of Legionella species and Legionella pneumophila by duplex PCR (dPCR) assay in cooling tower water samples from Jakarta, Indonesia.
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Date
2010-11
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Abstract
Aim: Since culture method is time-consuming and has low sensitivity, we developed a duplex PCR (dPCR) assay for the
detection of Legionella sp. and L. pneumophila in cooling tower samples. We used culture method as a gold standard.
Methods: Optimization of dPCR method was performed to obtain an assay with high sensitivity and specifi city. The
optimized method was used to detect Legionella sp. dan L. pneumophila in 9 samples obtained from 9 buildings in
Jakarta. For culture method, the bacteria were grown or isolated on selective growth factor supplemented-buffered
charcoal yeast extract (BCYE) media.
Results: Of 9 samples tested by dPCR assay, 6 were positive for Legionella species,1 was positive for L. pneumophila,
and 2 showed negative results. For the same samples, no Legionella sp. was detected by the culture method.
Conclusion: dPCR assay was much more sensitive than the culture method and was potentially used as a rapid,
specifi c and sensitive test for routine detection of Legionella sp. dan for L. pneumophila in water samples.
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Keywords
BCYE media, mip gene, 16S-rRNA gene
Citation
Yasmon Andi, Yusmaniar Yusmaniar, Karuniawati Anis, Bela Budiman. Simultaneous detection of Legionella species and Legionella pneumophila by duplex PCR (dPCR) assay in cooling tower water samples from Jakarta, Indonesia. Medical Journal of Indonesia. 2010 Nov; 19(4): 223-227.