Morphometric and molecular characterization of fungus Pestalotiopsis using nuclear ribosomal DNA analysis.
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Date
2012-09
Authors
Gehlot, Praveen
Singh, S K
Pathak, Rakesh
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Abstract
Taxonomy of the fungus Pestalotiopsis based on morphological characters has been equivocal. Molecular characterization of ten Pestalotiopsis species was done based on nuclear ribosomal DNA internal transcribed spacer (ITS) amplifications. Results of the analyses showed that species of genus Pestalotiopsis are monophyletic. We report ITS length variations, single nucleotide polymorphisms (SNPs) and insertions/deletions (INDELS) among ten species of Pestalotiopsis that did not cause any phylogenetic error at either genus or species designation levels. New gene sequences have been assigned (Gen Accession numbers from HM 190146 to HM 190155) by the National Centre for Biotechnology Information, USA.
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Keywords
Pestalotiopsis, Morphology, Host association, Molecular phylogeny, rDNA sequencing
Citation
Gehlot Praveen, Singh S K, Pathak Rakesh. Morphometric and molecular characterization of fungus Pestalotiopsis using nuclear ribosomal DNA analysis. Journal of Environmental Biology. 2012 Sept; 33(5): 897-901.