Evaluation of Tribulus terrestris Linn (Zygophyllaceae) Acetone extract for Larvicidal and Repellence Activity against Mosquito Vectors.

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Date
2008-12
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Abstract
Acetone extracts of leaves and seeds from the Tribulus terrestris (Zygophyllaceae) were tested against mature and immature different mosquito vectors under laboratory condition. The extract showed strong larvicidal, properties 100 per cent mortality in the 3rd-instar larvae was observed in the bioassays with An. culicifacies Giles species A, An. stephensi Liston , Culex quinquefasciatus Say and Aedes aegypti Linn ,against 200ppm of the leaf acetone extract and 100 ppm seed acetone extract. The LC values 50 of leaf acetone extract estimated for 3rd-instars An. culicifacies species A, An. stephensi, Cx. quinquefasciatus and Ae. aegypti after 24 hour of exposure were 117, 124, 168 and 185 ppm respectively. The LC values of seed 50 acetone extract estimated for 3rd-instars An. culicifacies species A, An. stephensi, Cx. quinquefasciatus and Ae. aegypti after 24 hour of exposure were 100, 72, 91 and 91 ppm respectively. It is confirmed from the LC values 50 that the seed acetone extract of T. terrestris is more effective compared to leaf extracts. A significant (P<0.004) higher concentration of acetone extract leaf was required to kill equal number of larvae i.e. against acetone extract of seed. The seed acetone extract showed strong repellent activity against adults mosquitoes. Per cent protection obtained against Anopheles culicifacies species A 100% repellency in 1h, 6h; Anopheles stephensi 100% repellency in 0h, 4h, 6h; and Culex quinquefasciatus 100% repellency in 0h, 2h, 4h, at 10% concentration respectively. Against Deet- 2.5% An. culicifacies Giles species A has shown 100% repellency in 1h,2h,6h , An. stephensi Liston 99% repellency in 4h , and Culex quinquefasciatus Say has shown 100% repellency in 1h,2h.
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Keywords
Malaria vector, Tribulus terrestris, Larvicidal, Repellency, DEET
Citation
Singh S P, Raghavendra K, Singh R K, Mohanty S S, Dash A P. Evaluation of Tribulus terrestris Linn (Zygophyllaceae) Acetone extract for Larvicidal and Repellence Activity against Mosquito Vectors. Journal of Communicable Diseases. 2008 Dec; 40(4): 255-261.