Simultaneous UV-Spectrophotometric estimation of Diclofenac and Tolperisone Hydrochloride in Tablet Dosage Form.
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Date
2013-08
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Abstract
Diclofenac sodium is a non-steroidal anti-inflammatory drug (NSAID) taken to reduce inflammation and as an analgesic reducing pain in certain
conditions. Tolperisone hydrochloride is a piperidine derivative, is a centrally-acting muscle relaxant. Two simple, accurate and economic methods; Q analysis and
first order derivative method have been described for the simultaneous spectrophotometric estimation of Diclofenac sodium and Tolperisone hydrochloride in tablet
dosage form. Absorption maxima of Diclofenac sodium and Tolperisone hydrochloride in distilled water were found to be 275.0 nm and 260.0 nm respectively.
Beer’s law was obeyed in the concentration range 5-50 μg/ml for Diclofenac and 5-60 μg/ml for Tolperisone hydrochloride. In Q analysis method, absorbances
were measured at the selected wavelengths, 237.0 nm (isoabsorptive point) and 260.0 nm (λmax of Tolperisone). In first order derivative method, zero crossing
point of Diclofenac sodium and Tolperisone hydrochloride were selected at 275.0 nm and 260.0 nm respectively. The analysis of binary pharmaceutical formulation
was carried by both methods. Results of two methods were validated statistically by recovery studies and were found to be satisfactory.
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Keywords
Diclofenac sodium (DIC), Tolperisone hydrochloride (TOL), Ultraviolet spectrophotometry, Q - Analysis method and First order method
Citation
Mahaparale S P, Shinde S S, Nirmal P N. Simultaneous UV-Spectrophotometric estimation of Diclofenac and Tolperisone Hydrochloride in Tablet Dosage Form. International Journal of Research and Development in Pharmacy and Life Sciences. 2013 Aug-Sept; 2(5): 574-579.