Improvised microinjection technique for mosquito vectors.
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Date
2012-12
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Abstract
Background & objectives: Bio-manipulation technique is of primary importance during the development of transgenic mosquitoes. The study describes the variable factors that influence the viability of medically important mosquito vectors during microinjection.
Methods: Three mosquito vectors belonging to the genus Aedes, Anopheles and Culex were microinjected at different developmental stages of their life cycle viz., egg, larvae, pupae and adult.
Results: The improvisations revealed an increased survivability of biomanipulated mosquitoes during the embryonic and adult microinjection. The study of injecting larvae and pupae resulted in poor survivability.
Interpretation & conclusions: The microinjection protocol was successfully tested on three important mosquito vectors. The critical period after biomanipulation which contributes heavily for the survivability factor was evaluated. The results provide a common protocol for biomanipulation of three mosquito vectors with enhanced survivability.
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Keywords
Bio-manipulation, critical period, microinjection, mosquito vectors, survivability
Citation
Kumar S Sampath, Puttaraju H P. Improvised microinjection technique for mosquito vectors. Indian Journal of Medical Research. 2012 Dec; 136(6): 971-978.