Hydrogen peroxide-scavenging enzymes impart tolerance to high temperature induced oxidative stress in sugarcane.
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Date
2012-05
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Abstract
Seventy-one genotypes of sugarcane from diverse agro-climatic zones of India viz. peninsular, northwest,
north-central and eastern zones, were screened for their tolerance to high temperature stress based on the
damage to leaf biomass i.e. necrosis of leaf-tips and margins, and rolling of leaves. Nine selected genotypes
showing variable response to heat injury were tested for activity pattern of isoforms of two H2O2-scavenging
enzymes; ascorbate peroxidase (APX) and catalase (CAT), under high temperature induced oxidative stress.
Changes in the activity of APX and CAT isozymes in leaves corresponded to the level of tolerance of
genotypes towards heat injury which was substantiated by the highly negative correlation coefficients of heat
injury levels of leaves vs. integrated density of APX and CAT isozyme bands. This indicated that the criteria
of higher expression of CATs’ and APXs’, the two major reactive oxygen species scavenging proteins in
leaves may be used to screen large seedling populations and germplasm for high temperature tolerance.
Description
Keywords
Saccharum spp. (hybrids), H2O2-scavengers, Heat tolerance, Oxidative stress and Reactive oxygen species
Citation
Srivastava Sangeeta, Pathak Ashwini Dutt, Gupta Prashant Shekhar, Shrivastava Ashok Kumar, Srivastava Arun Kumar. Hydrogen peroxide-scavenging enzymes impart tolerance to high temperature induced oxidative stress in sugarcane. Journal of Environmental Biology. 2012 May; 33(3): 657-661.