Size exclusion chromatography for the removal of pigments from extracellular ligninolytic enzyme extracts from decayed wheat straw.
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Date
2011-11
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Abstract
Solid-state fermentation of wheat straw was carried out by a native white rot basidiomycete Daedaleopsis
flavida strain 5A. Extract prepared from the 12-day decayed wheat straw contained extracellular ligninolytic
enzymes like manganese peroxidase (MnP), manganese-independent peroxidase (MIP), lignin peroxidase
(LiP) and laccase along with straw-degraded products and pigments. Sephacryl S-200 size exclusion
chromatography in 16/100 column was used for the separation of these ligninolytic enzymes and strawdegraded
products and pigments. Recovery of pigment-free ligninolytic enzyme activities as protein was
40% of the total proteins loaded and specific LiP activity increased 34 fold after size exclusion
chromatography. Thus accurate estimation of LiP by veratryl alcohol oxidation assay was possible only
after the removal of interfering pigments. The reproducibility of size exclusion chromatography is adjudged
satisfactory from the consistent results obtained after seven repetitive uses of matrices.
Description
Keywords
Daedaleopsis flavida, Size exclusion chromatography, Extracellular ligninolytic enzymes, Lignin peroxidase, Pigments, Solid-state fermentation
Citation
Shukla Dharmendra, Patel Bhavesh, Modi Hasmukh, Vyas Bharat Rajiv Manuel. Size exclusion chromatography for the removal of pigments from extracellular ligninolytic enzyme extracts from decayed wheat straw. Journal of Environmental Biology. 2011 Nov; 32(6): 713-717.