Denaturing gradient gel electrophoresis profiling of bacterial communities composition in Arabian Sea.
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Date
2011-05
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Abstract
Denaturing gradient gel electrophoresis (DGGE) was used to elucidate spatial and temporal variations in
bacterial community composition (BCC) from four locations along the central west coast of India. DNA extracts
from 36 water samples collected from surface, mid-depth (~10 m) and close to bottom (~20 m) during premonsoon,
postmonsoon, monsoon were analyzed by PCR for amplifying variable region of 16S rRNA gene and subsequently
through DGGE. Prominent bands were excised, cloned and sequenced indicated the preponderance of
gammaproteobacteria, bacteroidetes and cyanobacteria. Non-metric dimensional scaling of the DGGE gels
indicated that the spatial variations in BCC were prominent among the sampling locations. Temporal variations
in the BCC appear to be influenced by monsoonal processes. The canonical correspondence analyses suggest
that the concentration of chlorophyll a and nitrate are two important environmental factors for both spatial and
temporal variations in BCC. Chlorophyll a seems to be impart a top-down control of BCC while nitrate, the
bottom-up control. Our results also suggest that BCC can vary over a small geographic distance in highly
dynamic, seasonally predisposed tropical coastal waters.
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Keywords
Arabian sea, Bacterial community, DGGE, Spatio-temporal variations, Central west-coast of India
Citation
Singh Sanjay Kumar, Ramaiah Nagappa. Denaturing gradient gel electrophoresis profiling of bacterial communities composition in Arabian Sea. Journal of Environmental Biology. 2011 May; 32(3): 339-346.