Reliability of solid phase microextraction in estimating bioavailability of pyrene in soil.
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Date
2011-05
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Abstract
Solid phase microextraction (SPME) coupled with gas chromatography was employed to estimate bioavailability
of pyrene in soils with different properties of textures, organic matter contents (SOM) and aging periods.
Experimental results indicated that biodegradation rates increased from 0.10 (sandy loam) to 0.15 (silty loam)
μg g-1 hr-1. By contrast, biodegradation rate decreased from 0.10 (1.3% SOM) to 0.04 (7.6% SOM) μg g-1 hr-1.
The amounts of pyrene biodegraded decreased 27% when SOM was modified from 1.3 to 7.6%, indicating that
distributions of pyrene in soils at biodegradation end points were affected by the SOM. Sequestration as
measured by sonication extraction had evidently occurred in aged soil samples. SPME measurements slightly
overestimated the amount of pyrene degraded by indigenous and seeded microorganisms, in soils with the
different properties (correlation coefficient, R2 = 0.74). The present study demonstrates that the SPME method
can not replace biodegradation tests commonly used for predicting bioremediation efficacy.
Description
Keywords
SPME, Gas chromatography, Bioavailability, Pyrene, Soil
Citation
Liu Hsiang-Chao, Hwu Ching-Shyung, Hung Jui-Min, Lai Hung-Yu, Chang Hung-Yueh, Lu Chih-Jen. Reliability of solid phase microextraction in estimating bioavailability of pyrene in soil. Journal of Environmental Biology. 2011 May; 32(3): 319-323.