Screening and detection of biomarkers in chickpea plants exposed to chromium and cadmium.
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Date
2011-01
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Abstract
A broad screening protocol, covering the most general phytochemical groups of compounds, was developed on
the basis of high performance thin layer chromatography (HPTLC). A total of six TLC systems, comprising three
derivatization reagents, two stationary phases and two mobile phases, were included. The screening method
was applied for the identification of biomarkers in the chickpea plant exposed to cadmium and chromium. The
biomarkers were selected on the basis of significant changes (0.26-4.6 fold) in concentration levels of
phytochemicals. Totally, five different amino acids, three organic acids, one sulphur containing compound and
one sugar were identified as biomarkers in chickpea exposed heavy metal.
Description
Keywords
Screening, Biomarker, Phytochemical, Chickpea, Thin layer chromatography
Citation
Khan Mather Ali, Ram Mauji, Jha Prabhakar, Ahmad M Mobeen, Alam Pravej, Kamaluddin, Ali Athar, Kiran Usha, Abdin M Z. Screening and detection of biomarkers in chickpea plants exposed to chromium and cadmium. Journal of Environmental Biology. 2011 Jan; 32(1): 51-55.