Antibacterial activity of house fly-maggot extracts against MRSA (Methicillin-resistant Staphylococcus aureus) and VRE (Vancomycin-resistant enterococci).
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Date
2010-09
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Abstract
The principal objective of this study was to determine the in vitro antibacterial activity of the water-soluble protein enzymatic
hydrolysates and the ethanol (EtOH)-extracted fraction obtained from fly-maggots (Musca domestica L.) against MRSA (methicillinresistant
Staphylococcus aureus) strains and VRE (Vancomycin-resistant enterococci) 5117 strain. The water soluble protein enzymatic
hydrolysates were prepared via 4 or 8 hr of incubation after mixing with the water-soluble protein extracted from the fly-maggots plus
thermolysin. The EtOH-extracts A was prepared by homogenizing after mixing with the fly-maggots plus pure EtOH at room temperature.
The EtOH-extracts B and C was obtained via filtering after stationing for 24 hr at 4-20oC using the EtOH-extracts A, respectively. The
growth inhibition curves for MRSA strain 3595 and VRE strain 5117 in the water-soluble protein enzymatic hydrolysates were increased and
evidenced concentration-dependent inhibition in the 8-hr hydrolysate as compared with the 4-hr hydrolysate (p<0.05). The growth inhibition
curves for MRSA and VRE strains in the EtOH-extracted fraction obtained from the fly-maggots were high in EtOH-extract C as compared
with EtOH-extracts A and B (p<0.05). The minimum inhibitory concentrations (MIC) in the EtOH-extracts C, in which the growth inhibition
of MRSA and VRE strains was increased, were determined to be 40, 50, 50, 60, 40 and 60 μg ml-1 in MRSA strains 3598, 3595, 3601, 3589,
3597 and 3595, respectively. While the butanol fraction obtained from EtOH extract C evidenced profound antibacterial activity against the
MRSA and VRE strains, the antibacterial activity of the hexane, ethyl acetate, and water layers could not be adequately confirmed.
Description
Keywords
Maggot extracts, MRSA, Antibacterial activity, Minimum inhibitory concentration
Citation
Park Sang O, Shin Jae Hong, Choi Won Keun, Park Byung Sung, Oh Jin Seok, Jang Aera. Antibacterial activity of house fly-maggot extracts against MRSA (Methicillin-resistant Staphylococcus aureus) and VRE (Vancomycin-resistant enterococci). Journal of Environmental Biology. 2010 Sept; 31(5): 865-871.