Biodegradation of phenol by native microorganisms isolated from coke processing wastewater.
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Date
2010-05
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Abstract
The present investigation was undertaken to assess the biodegradation of phenol by native bacteria strains isolated from coke
oven processing wastewater. The strains were designated ESDSPB1, ESDSPB2 and ESDSPB3 and examined for colony morphology
Gram stain characters and biochemical tests. Phenol degrading performance of all the strains was evaluated initially. One of the strains
namely ESDSPB2 was found to be highly effective for the removal of phenol, which was used as sole carbon and energy source. From an
initial concentration of 200 mg l-1 it degraded to 79.84 ± 1.23 mg l-1. In turn the effect of temperature (20 to 450C), pH (5 – 10) and glucose
concentration (0, 0.25 and 0.5%) on the rate of phenol degradation by that particular strain was investigated. Observations revealed that
the rate of phenol biodegradation was significantly affected by pH, temperature of incubation and glucose concentration. The optimal
conditions for phenol removal were found to be pH of 7 (84.63% removal), temperature, 300C (76.69% removal) and 0.25% supplemented
glucose level (97.88% removal). The main significance of the study is the utilization of native bacterial strains from the waste water itself
having potential of bioremediation.
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Keywords
Bacterial strains, Phenol degradation, pH, Temperature, Glucose
Citation
Chakraborty S, Bhattacharya T, Patel T N, Tiwari K K. Biodegradation of phenol by native microorganisms isolated from coke processing wastewater. Journal of Environmental Biology. 2010 May; 31(3): 293-296.