Activity of phosphatases in the hemocytes of estuarine edible mudcrab, Scylla serrata exposed to arsenic.
Loading...
Date
2009-09
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Sundarbans biosphere reserve is an ecologically important area of West Bengal, which supports a wide variety of
estuarine species including Scylla serrata estuarine mudcrab of economic value. In this present study, activity of phosphatases
(acid and alkaline) of hemocytes was estimated under the sublethal exposure of arsenic in controlled static water environment for
1, 2, 3, 4, 7 and 15 days of exposure. Exposure to 3 ppm of sodium arsenite for 15 days, resulted a maximum inhibition of activity
of acid phosphatase (ACP) as 0.016 .M mg-1 protein min-1 with intermediate activity in different spans of exposure against the
control value of 1.83 .M mg-1 protein min-1. Similar mode of maximum inhibition of activity of alkaline phosphatase (ALP) was
recorded as 0.008 .M mg-1 protein min-1, when the crabs were exposed to 3 ppm of sodium arsenite for 15 days against the control
value of 0.694 .M mg-1 protein min-1. Exposure to 1 ppm of sodium arsenite caused inhibition of activity of ACP which ranged from
1.76 to 0.70 .M mg-1 protein min-1. For 2 and 3 ppm of exposure activity of ACP ranged from 1.12 to 0.42 and 0.67 to 0.016 .M mg-1
protein min-1 respectively. For the activity of ALP exposure to 2 and 3 ppm of sodium arsenite yielded an inhibition of activity which
ranged from 0.472 to 0.279 and 0.275 to 0.008 .M mg-1 protein min-1 respectively. Arsenic exposure resulted a dose dependent
decrease in the activities of both of the phosphatases and may be considered as a suitable biomarker of aquatic pollution in
Sundarbans mangrove.
Description
Keywords
Sodium arsenite, Scylla serrata, Hemocytes, Phosphatase, Sundarbans
Citation
Saha Sanjib, Ray Mitali, Ray Sajal. Activity of phosphatases in the hemocytes of estuarine edible mudcrab, Scylla serrata exposed to arsenic. Journal of Environmental Biology. 2009 Sept; 30(5): 655-658.