Characterization of a diesel-degrading strain isolated from a hydrocarbon-contaminated site.
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Date
2009-01
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Abstract
A diesel-degrading bacterium has been isolated from a diesel-polluted site. The isolate was tentatively identified as Staphylococcus
aureus strain DRY11 based on partial 16S rDNA molecular phylogeny and Biolog® GP microplate panels and Microlog® database. Isolate
11 showed an almost linear increase in cellular growth with respect to diesel concentrations with optimum growth occurring at 4% (v/v)
diesel concentration. Optimization studies using different nitrogen sources showed that the best nitrogen source was potassium nitrite.
Sodium nitrite was optimum at 1.2 g l-1 and higher concentrations were strongly inhibitory to cellular growth. The optimal pH that supported
growth of the bacterium was between 7.5 to 8.0 and the isolate exhibited optimal broad temperature supporting growth on diesel from 27
to 37 oC. An almost complete removal of diesel components was seen from the reduction in hydrocarbon peaks observed using Solid Phase
Microextraction Gas Chromatography analysis after 5 days of incubation. The characteristics of this bacterium suggest that it is suitable
for bioremediation of diesel spills and pollutions in the tropics.
Description
Keywords
Isolation, Characterization, Diesel-degrading, S. aureus
Citation
Shukor M Y, Dahalan F A, Jusoh A Z, Muse R, Shamaan N A, Syed M A. Characterization of a diesel-degrading strain isolated from a hydrocarbon-contaminated site. Journal of Environmental Biology. 2009 Jan; 30(1): 145-150.