Alkaline protease production, extraction and characterization from alkaliphilic Bacillus licheniformis KBDL4: A Lonar soda lake isolate.
Loading...
Date
2012-08
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
A bacterium producing an alkaline protease was isolated from the Lonar soda lake, Buldhana district (19°58' N; 76°31' E), Maharashtra, India. The most appropriate medium for the growth and protease production was composed of (g/L): casein 10; yeast extract 4; KH2PO4 0.5, K2HPO4 0.5 and CaCl2 0.5. The enzyme showed maximum activity with and without 5 mM Ca2+ at 70 and 60 oC, respectively. The enzyme retained 40 and 82% of its initial activity after heating for 60 min at 60 oC, in absence and presence of 5 mM CaCl2 respectively. The enzyme remained active and stable at pH 8-12, with an optimum at pH 10. The enzyme showed stability towards non-ionic and anionic surfactants, and oxidizing agents. It also showed excellent stability and compatibility with commonly used laundry detergents. Wash performance analysis revealed that enzyme could effectively remove blood stains. It also showed decomposition of gelatinous coating on X- ray film.
Description
Keywords
Alkaline protease, Bacillus licheniformis, Gelatinous coating, Detergent compatibility, Lonar soda lake
Citation
Pathak Anupama P, Deshmukh Kshipra B. Alkaline protease production, extraction and characterization from alkaliphilic Bacillus licheniformis KBDL4: A Lonar soda lake isolate. Indian Journal of Experimental Biology. 2012 Aug; 50(8): 569-576.