A novel method of plasmid isolation using laundry detergent.
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Date
2011-07
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Abstract
Since the discovery of plasmid, various methods have been developed to isolate plasmid DNA. All the methods have one common and important target of isolating plasmid DNA of high quality and quantity in less time. These methods are not completely safe because of use of toxic chemicals compounds. The developed protocol for plasmid extraction is based on the alkaline lysis method of plasmid preparation (extraction at pH 8.0) with slight modifications. Cell lysis reagent sodium dodecyl sulfate is replaced by lipase enzyme present in laundry detergent. A good plasmid preparation can be made, which is well suited for subsequent molecular biology applications. By taking safety measures on count, contaminants like, RNA and protein can be completely avoided with maximized plasmid yield. The resultant plasmid quality and quantity can be well comparable to other prevalent methods.
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Keywords
Plasmid, Laundry detergent, Vector
Citation
Yadav P, Yadav A, Garg V, Datta T K, Goswami S L, De S. A novel method of plasmid isolation using laundry detergent. Indian Journal of Experimental Biology. 2011 July; 49(7): 558-560.