Direct inoculation on Phoenix panels for identification and antimicrobial susceptibility from positive BACTEC cultures: First study from India.
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Date
2011-07
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Abstract
Purpose: This was a prospective study planned in a super-specialty hospital in Delhi to reduce turnaround times of identification-susceptibility results of positive blood cultures. Materials and Methods: One hundred consecutive single morphology non-duplicate cultures were inoculated on Becton Dickinson Phoenix™ panels by growth recovered directly from liquid BACTEC™ media and after pure growth on solid media. Results: Complete concordance was observed in 72.4% of gram-negative and 45.8% of gram-positive isolates. For gram-negative isolates, categorical agreement (CA) was >83% and essential agreement (EA) was >96% among all antibiotics tested, very major errors (VME) were 0.13%, major errors (ME) 0.54%, and minor errors (MiE) were 3.01%. For gram-positive isolates, VME was 0.73%, 1.10% MiE and no ME. It was observed that average time from receipt of specimen to release of reports was 30:34 h and 32 h for gram-negative and gram-positive isolates if reports of "Direct" panels were to be released. Conclusions: By direct panel inoculation, a decrease of at least 18-20 h in turnaround time was observed compared with the standard method. This helps early change to effective antibiotic therapy and also reduces the expenditure incurred for a patient's hospital stay by average Rs 20,000 ($443) per day.
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BACTEC, blood cultures, direct inoculation, Phoenix, panels
Citation
Duggal S, Jesaiwal S K, Tandon N, Chugh T D. Direct inoculation on Phoenix panels for identification and antimicrobial susceptibility from positive BACTEC cultures: First study from India. Indian Journal of Medical Microbiology. 2011 Jul-Sept; 29(3): 283-287.