Absence of Mycobacterium avium ss paratuberculosis-specific IS900 sequence in intestinal biopsy tissues of Indian patients with Crohn’s disease.
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Date
2009-09
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Abstract
Background and Objective The role of Mycobacterium avium ss paratuberculosis (MAP) in the etiopathology of Crohn’s
disease (CD) remains controversial, because of conflicting
reports demonstrating the presence of MAP-specific insertion
sequence from intestinal biopsy tissues of patients clinically
diagnosed for the disease. The present study was carried out to
investigate the presence of MAP DNA in the intestinal tissues
of CD patients to ascertain the relevance of MAP in Indian
patients with CD.
Methods Patients diagnosed as CD at our institute were
recruited. Healthy individuals without inflammatory bowel
disease served as controls. Mucosal biopsy specimens were
collected from ileum and colon in duplicates and subjected
to histopathological examination and polymerase chain
reaction (PCR) amplification. Total DNA (81 CD patients,
85 healthy individuals) and total RNA (12 CD patients, 12
healthy individuals) isolated from tissue specimens was used
for amplification of MAP-specific IS900 by nested PCR.
Results MAP-specific IS900 DNA and RNA could not be
detected by nested PCR in the intestinal tissues of any patient
with CD.
Conclusion Our results do not support the etiological role of MAP in the pathogenesis of CD in Indian patients.
Description
Keywords
Inflammatory bowel disease, MAP-specific IS900, Mycobacterium avium ss paratuberculosis
Citation
Sasikala Mitnala, Reddy D Nageshwar, Pratap Nitesh, Sharma Sanjeev Kumar, Reddy P Balkumar, Sekaran Anuradha, Banerjee Rupa, Reddy D Bhaskara. Absence of Mycobacterium avium ss paratuberculosis-specific IS900 sequence in intestinal biopsy tissues of Indian patients with Crohn’s disease. Indian Journal of Gastroenterology. 2009 Sept-Oct; 28(5): 169-174.